Is Bacillus cytotoxicus from edible insects a threat?

Bacillus cytotoxicus is considered a potential emerging foodborne pathogen that has been under investigation in recent years. Most studies have focused on strains from vegetables, particularly potato products, but there is limited information on strains from other food sources. This study addresses the current research gap by investigating the genomic and phenotypic features of B. cytotoxicus isolated from edible insects. The whole genomes and key phenotypic traits of 20 strains isolated from edible insects were investigated. The comparative genome analysis also included 44 available genomes from other sources to identify possible genetic links and the mosaicism of virulence profiles (VP) and antimicrobial resistance genes (AMR). B. cytotoxicus isolated from edible insects showed marked thermotolerance, when vegetative forms could grow at 50–60 °C and survive at 65 °C and exhibited marked proteolytic activities, even at higher temperatures. The heterogeneous phenotypes observed suggest...