Ionized Ti surfaces increase cell adhesion properties of mesenchymal stem cells

Titanium (Ti) surface topography of dental implants strongly influences osseointegration. To this view, in the present work we have analyzed the influence of an ionized titanium surface on stem cell adhesion properties. Human Dental Pulp Stem Cells (DPSCs) has been used in order to evaluate the influence of the ionized surface to the osteogenic commitment, extracellular matrix component production and cell adhesion molecules. The morphology of the cells grown onto this surface has been analyzed with SEM and the safety of the surface has been tested by means of karyotype analysis, by AMES test and by hemocompatibility assay. Results confirmed that at 25 days of cultures DPSCs show a substantial expression of some osteoblast specific markers and a strong increase of cell adhesion molecules.