Molecularly designed, random copolymer-bioconjugates based on poly(2-methyl-2-oxazoline) (PMOXA) and poly(2-ethyl-2-oxazoline) (PEOXA) are crosslinked in the presence of sortase A (SA) and human articular chondrocytes (hACs), to yield cellularized polymer networks. Their gelation kinetics and mechanical properties are finely tuned by varying the concentration of SA, while a cell viability >90% is achieved after several weeks of culture, even in the absence of any cell-adhesive cue.
Enzymatically crosslinked poly(2-alkyl-2-oxazoline) networks for 3D cell culture
Benetti E
2018
Abstract
Molecularly designed, random copolymer-bioconjugates based on poly(2-methyl-2-oxazoline) (PMOXA) and poly(2-ethyl-2-oxazoline) (PEOXA) are crosslinked in the presence of sortase A (SA) and human articular chondrocytes (hACs), to yield cellularized polymer networks. Their gelation kinetics and mechanical properties are finely tuned by varying the concentration of SA, while a cell viability >90% is achieved after several weeks of culture, even in the absence of any cell-adhesive cue.
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