Since 1996 a new Infectious Bronchitis virus (IBV) genotype, referred to as Q1, circulated in China and was reported for the first time in Italy in 2011. A deep molecular investigation of a Q1 IBV strain isolated in Italy in 2013 has raised hypotheses regarding the origin of this genotype. The phylogenetic analysis of the S1 gene sequence of the Q1 Italian strain revealed, beyond an obvious correlation with Q1 sequences available in web databases, a close relationship with sequences of the 624I strains circulating in Italy in the early ‘90s. The close genetic correlation observed led to the hypothesis that 624I was an ancestor of the Q1 genotype. Despite the fact that most heterogeneity of IBVs occurs in the S1 gene, the sequence analysis of this single gene was not sufficient neither to confirm nor deny this hypothesis. For further investigation, in the present study an Italian 624I strain was for the first time fully sequenced and phylogenetic analysis was performed using complete IBV genome sequences available on database ViPR. The possible recombinant nature of the sequenced strain was evaluated using SimPlot analysis.
New findings on the genetic correlation between Q1 and 624I genotypes of Infectious Bronchitis Virus.
Laconi, A.;FRANZO, GIOVANNI;CECCHINATO, MATTIA;
2017
Abstract
Since 1996 a new Infectious Bronchitis virus (IBV) genotype, referred to as Q1, circulated in China and was reported for the first time in Italy in 2011. A deep molecular investigation of a Q1 IBV strain isolated in Italy in 2013 has raised hypotheses regarding the origin of this genotype. The phylogenetic analysis of the S1 gene sequence of the Q1 Italian strain revealed, beyond an obvious correlation with Q1 sequences available in web databases, a close relationship with sequences of the 624I strains circulating in Italy in the early ‘90s. The close genetic correlation observed led to the hypothesis that 624I was an ancestor of the Q1 genotype. Despite the fact that most heterogeneity of IBVs occurs in the S1 gene, the sequence analysis of this single gene was not sufficient neither to confirm nor deny this hypothesis. For further investigation, in the present study an Italian 624I strain was for the first time fully sequenced and phylogenetic analysis was performed using complete IBV genome sequences available on database ViPR. The possible recombinant nature of the sequenced strain was evaluated using SimPlot analysis.
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