IMPORTANCE A clearer definition of the role of neurofilament light chain (NFL) as a biomarkerin amyotrophic lateral sclerosis (ALS) is needed.OBJECTIVES To assess the ability of NFL to serve as a diagnostic biomarker in ALS and theprognostic value of cerebrospinal fluid NFL in patients with ALS.DESIGN, SETTING, AND PARTICIPANTS In this single-center, retrospective, longitudinal study,disease progression was assessed by the ALS Functional Rating Score–Revised and the ALSMilano-Torino Staging system at baseline and 6, 12, 24, and 36 months. Cerebrospinal fluidsamples were obtained from 176 patients admitted to the Department of Neurosciences of the University of Padua, Padova, Italy, from January 1, 2010, through February 29, 2016.Patients with ALS underwent ambulatory follow-up at the same department. MAIN OUTCOMES AND MEASURES Levels of NFL. RESULTS The study included 94 patients with ALS (64 men [36.4%] and 30 women [17.0%];median age, 62.5 years), 20 patients with frontotemporal dementia (FTD) (8 men [4.5%] and12 women [6.8%]; median age, 65 years), 18 patients with motor neuropathies (14 men[8.0%] and 4 women [2.3%]; median age, 63 years), and 44 controls (24 men [13.6%] and 20women [11.4%]; median age, 54 years). Log-transformed NFL (log[NFL]) concentrations werehigher in the ALS and FTD groups compared with the motor neuropathies and control groups(hazard ratio [HR], 2.45; 95% CI, 1.66-3.61; P < .001). Patients with typical ALS (HR, 1.0, progressive bulbar palsy (HR, 1.48; 95% CI, 0.58-3.75; P = .41), and upper motor neuron dominant ALS (HR, 0.12; 95% CI, 0.02-0.61; P = .01) had higher levels of NFL than did those with flail arm or leg syndrome (HR, 0.28; 95% CI, 0.08-0.10; P = .049) and progressive muscular atrophy (HR, 0.17; 95% CI, 0.22-1.36; P = .10). There was an inverse correlation between log[NFL] concentration and overall survival (HR, 2.45; 95% CI, 1.66-3.61; P < .001). There was no evidence of different log[NFL] concentrations and survival in genetic ALS. CONCLUSIONS AND RELEVANCE This study confirms the role of NFL as a biomarker in ALS. Elevation in NFL levels in patients with upper motor neuron involvement and FTD might reflect the corticospinal tract degeneration. Low NFL levels in patients with lower motor neuron signs might be a prognostic indicator of milder phenotypes of disease
Diagnostic and Prognostic Biomarkers in Amyotrophic Lateral Sclerosis: Neurofilament Light Chain Levels in Definite Subtypes of Disease
GAIANI, ALESSANDRA;BELLO, LUCA;QUERIN, GIORGIA;PUTHENPARAMPIL, MARCO;RUGGERO, SUSANNA;TOFFANIN, ELISABETTA;CAGNIN, ANNACHIARA;BRIANI, CHIARA;PEGORARO, ELENA;SORARU', GIANNI
2017
Abstract
IMPORTANCE A clearer definition of the role of neurofilament light chain (NFL) as a biomarkerin amyotrophic lateral sclerosis (ALS) is needed.OBJECTIVES To assess the ability of NFL to serve as a diagnostic biomarker in ALS and theprognostic value of cerebrospinal fluid NFL in patients with ALS.DESIGN, SETTING, AND PARTICIPANTS In this single-center, retrospective, longitudinal study,disease progression was assessed by the ALS Functional Rating Score–Revised and the ALSMilano-Torino Staging system at baseline and 6, 12, 24, and 36 months. Cerebrospinal fluidsamples were obtained from 176 patients admitted to the Department of Neurosciences of the University of Padua, Padova, Italy, from January 1, 2010, through February 29, 2016.Patients with ALS underwent ambulatory follow-up at the same department. MAIN OUTCOMES AND MEASURES Levels of NFL. RESULTS The study included 94 patients with ALS (64 men [36.4%] and 30 women [17.0%];median age, 62.5 years), 20 patients with frontotemporal dementia (FTD) (8 men [4.5%] and12 women [6.8%]; median age, 65 years), 18 patients with motor neuropathies (14 men[8.0%] and 4 women [2.3%]; median age, 63 years), and 44 controls (24 men [13.6%] and 20women [11.4%]; median age, 54 years). Log-transformed NFL (log[NFL]) concentrations werehigher in the ALS and FTD groups compared with the motor neuropathies and control groups(hazard ratio [HR], 2.45; 95% CI, 1.66-3.61; P < .001). Patients with typical ALS (HR, 1.0, progressive bulbar palsy (HR, 1.48; 95% CI, 0.58-3.75; P = .41), and upper motor neuron dominant ALS (HR, 0.12; 95% CI, 0.02-0.61; P = .01) had higher levels of NFL than did those with flail arm or leg syndrome (HR, 0.28; 95% CI, 0.08-0.10; P = .049) and progressive muscular atrophy (HR, 0.17; 95% CI, 0.22-1.36; P = .10). There was an inverse correlation between log[NFL] concentration and overall survival (HR, 2.45; 95% CI, 1.66-3.61; P < .001). There was no evidence of different log[NFL] concentrations and survival in genetic ALS. CONCLUSIONS AND RELEVANCE This study confirms the role of NFL as a biomarker in ALS. Elevation in NFL levels in patients with upper motor neuron involvement and FTD might reflect the corticospinal tract degeneration. Low NFL levels in patients with lower motor neuron signs might be a prognostic indicator of milder phenotypes of disease
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