Pterodon emarginatus Vogel (Fabaceae) is a plant from the Cerrado which is commonly known as 'sucupira branca' or 'faveiro'. It is widely used in Brazilian folk medicine for the treatment of rheumatism, sore throats and back problems. Its seeds are commercially available as they are largely used for their properties, mainly as anti-inflammatory remedy (de Moraes et al., 2012). Thus, the aim of this research was the study of Pterodon emarginatus seed extracts and essential oil, also to improve their use. For this, we performed different sets of protocols: a) the gas chromatography coupled with mass spectrometry (GC/MS) analysis to determine the sesquiterpenoid content; b) two radical-scavenging assays to determine the antioxidant activity, and 3) the MTT assay on colorectal adenocarcinoma cell line (HT29 cells) to evaluate cellular viability. Thus, the antioxidant activity was evaluated using assays based on two different mechanisms: the Oxygen Radical Absorbance Capacity (ORAC) assay, based on HAT reaction, and DPPH radical scavenging capacity assay, based on SET reaction. The DPPH assay was performed according to the method reported by Brand-Williams et al. (Brand- Williams, 1995) with some modifications (Chen et al., 2013). The ORAC assay was applied according to the method of Gillespie et al. (Gillespie, 2007), appropriately modified (Bertin et al., 2013). We also determined the Total Phenolic Content (TPC) and the Total Flavonoid Content (TFC) for each extract because these compounds are generally related to the antioxidant properties of plants. The Pterodon emarginatus seeds were collected from May to August 2013, near the city of Guanambi, Bahia State, Brazil. The ethanolic extract (TME, Traditional Medicinal Extract), the sequential extracts with different solvents (hexane, chloroform, ethyl-acetate and methanol) and the essential oil (EO) were obtained. The GC analysis of Pterodon emarginatus extracts showed the presence of β-caryophyllene and farnesol, which were chosen as reference compounds in our research.
Pterodon emarginatus Vogel, a Brazilian medicinal plant: antioxidant properties and studies on viability of HT29 cells
DONATI, MADDALENA;MONDIN, ANDREA;MONTOPOLI, MONICA;PASTORE, PAOLO;CAPARROTTA, LAURA;FROLDI, GUGLIELMINA
2015
Abstract
Pterodon emarginatus Vogel (Fabaceae) is a plant from the Cerrado which is commonly known as 'sucupira branca' or 'faveiro'. It is widely used in Brazilian folk medicine for the treatment of rheumatism, sore throats and back problems. Its seeds are commercially available as they are largely used for their properties, mainly as anti-inflammatory remedy (de Moraes et al., 2012). Thus, the aim of this research was the study of Pterodon emarginatus seed extracts and essential oil, also to improve their use. For this, we performed different sets of protocols: a) the gas chromatography coupled with mass spectrometry (GC/MS) analysis to determine the sesquiterpenoid content; b) two radical-scavenging assays to determine the antioxidant activity, and 3) the MTT assay on colorectal adenocarcinoma cell line (HT29 cells) to evaluate cellular viability. Thus, the antioxidant activity was evaluated using assays based on two different mechanisms: the Oxygen Radical Absorbance Capacity (ORAC) assay, based on HAT reaction, and DPPH radical scavenging capacity assay, based on SET reaction. The DPPH assay was performed according to the method reported by Brand-Williams et al. (Brand- Williams, 1995) with some modifications (Chen et al., 2013). The ORAC assay was applied according to the method of Gillespie et al. (Gillespie, 2007), appropriately modified (Bertin et al., 2013). We also determined the Total Phenolic Content (TPC) and the Total Flavonoid Content (TFC) for each extract because these compounds are generally related to the antioxidant properties of plants. The Pterodon emarginatus seeds were collected from May to August 2013, near the city of Guanambi, Bahia State, Brazil. The ethanolic extract (TME, Traditional Medicinal Extract), the sequential extracts with different solvents (hexane, chloroform, ethyl-acetate and methanol) and the essential oil (EO) were obtained. The GC analysis of Pterodon emarginatus extracts showed the presence of β-caryophyllene and farnesol, which were chosen as reference compounds in our research.
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