The productivity of sugar beet is strongly limited by several biotic stresses. These include rhizomania (induced by beet necrotic yellow vein virus, BNYVV) that causes yield losses of 20-50% or more. The only way to control this disease is the use of resistant varieties. Rz1 is the major resistance gene present within commercial sugar beet varieties. A linkage analysis was carried out on F2 individuals to identify single nucleotide polymorphism (SNP) markers linked to Rz1. From the 384 SNPs evaluated, three SNPs (SNP1, SNP2 and SNP3) resulted tightly linked to Rz1. Linkage analysis confirms that the Rz1 gene is located on chromosome 3 and is flanked by two closely linked markers named SNP1 and SNP2 with genetic distances of 0.7 cM and 5.2 cM, respectively. The SNP3 is located at 8.9 cM. These first reported SNPs provide a valuable basis for marker assisted introgression of Rz1 resistance to rhizomania.
Identification of SNP markers linked to the Rz1 gene in sugar beet
STEVANATO, PIERGIORGIO;TREBBI, DANIELE;BROCCANELLO, CHIARA;SACCOMANI, MASSIMO
2012
Abstract
The productivity of sugar beet is strongly limited by several biotic stresses. These include rhizomania (induced by beet necrotic yellow vein virus, BNYVV) that causes yield losses of 20-50% or more. The only way to control this disease is the use of resistant varieties. Rz1 is the major resistance gene present within commercial sugar beet varieties. A linkage analysis was carried out on F2 individuals to identify single nucleotide polymorphism (SNP) markers linked to Rz1. From the 384 SNPs evaluated, three SNPs (SNP1, SNP2 and SNP3) resulted tightly linked to Rz1. Linkage analysis confirms that the Rz1 gene is located on chromosome 3 and is flanked by two closely linked markers named SNP1 and SNP2 with genetic distances of 0.7 cM and 5.2 cM, respectively. The SNP3 is located at 8.9 cM. These first reported SNPs provide a valuable basis for marker assisted introgression of Rz1 resistance to rhizomania.
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