The 3d hyaluronan based scaffolds were utilized for in-vitro cultures of rat sciatic nerve cells. The hyaluronan derived basic material was a 100% benzyl ester of hyaluronic acid referred to as HYAFF-11 which includes non woven mesh made of 20μm-thick fibers and tubes made of 20μm-thick interwoven fibers. Cell containing biomaterial scaffolds were embedded in OCT, submerged in iso-penane and snap-frozen in liquid nitrogen and the sliced into section of 5 μm. The results show that the HYADD-11 is suitable biomaterial that can support in vitro nerve cell growth in particular several devices of biomaterial were able to facilitate nerve fragment adhesion and the outgrowth of neural cells.
Utilization of 3d hyaluronan based scaffolds for In Vitro cultures of rat sciatic nerve cells
ZAVAN, BARBARA;CORTIVO, ROBERTA;BRUN, PAOLA;ABATANGELO, GIOVANNI
2004
Abstract
The 3d hyaluronan based scaffolds were utilized for in-vitro cultures of rat sciatic nerve cells. The hyaluronan derived basic material was a 100% benzyl ester of hyaluronic acid referred to as HYAFF-11 which includes non woven mesh made of 20μm-thick fibers and tubes made of 20μm-thick interwoven fibers. Cell containing biomaterial scaffolds were embedded in OCT, submerged in iso-penane and snap-frozen in liquid nitrogen and the sliced into section of 5 μm. The results show that the HYADD-11 is suitable biomaterial that can support in vitro nerve cell growth in particular several devices of biomaterial were able to facilitate nerve fragment adhesion and the outgrowth of neural cells.
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