Background and Aim: An increased susceptibility of LDL to oxidation has been related to the genesis of vascular disease. The purpose of this study was to investigate whether this is atherogenic in patients with carotid atherosclerosis and free from other major risk factors. Methods: Seventy-two subjects undenvent echo-color-Doppler examination of their carotid arteries. None were hypertensive, hyperlipidemic, diabetic or obese, nor suffered from major chronic diseases. Forty were free from carotid lesions (Controls), whereas thirty-two subjects had evidence of carotid atherosclerosis (Cases). Fourteen young healthy individuals acted as a further control (Young controls). The susceptibility of LDL to oxidation was tested in vitro by monitoring the kinetics of conjugated diene formation induced by the free radical generator 2,2′-azobis (amidinopropane) dihydrochloride (AAPH).Their fatty acid composition and the vitamin E content were also measured. Results: The results showed a significantly greater peroxidation rate in cases than in controls (p-0.003), whereas no significant difference was found for the lag phase. The peroxidation rate for the whole population was significantly correlated with the LDL polyunsaturated fatty acid content (r=0.31, p=0.01). An inverse correlation was also observed between this rate and the 18:1/18:2 ratio in LDL (r=-0.36, p=0.004). A longer lag phase and a higher LDL vitamin E content were found in young controls as compared with the other two groups (p<0.0001). Conclusions: The study suggests that LDL oxidation might play a significant role in atherogenesis. It also indicates that the fatty acid composition of LDL may determine their in vivo oxidizability. © 1998, Medikal Press.
Increased susceptibility of LDL to oxidation in patients with carotid atherosclerosis
Lusiani L.;Pesavento R.;Pagnan A.
1998
Abstract
Background and Aim: An increased susceptibility of LDL to oxidation has been related to the genesis of vascular disease. The purpose of this study was to investigate whether this is atherogenic in patients with carotid atherosclerosis and free from other major risk factors. Methods: Seventy-two subjects undenvent echo-color-Doppler examination of their carotid arteries. None were hypertensive, hyperlipidemic, diabetic or obese, nor suffered from major chronic diseases. Forty were free from carotid lesions (Controls), whereas thirty-two subjects had evidence of carotid atherosclerosis (Cases). Fourteen young healthy individuals acted as a further control (Young controls). The susceptibility of LDL to oxidation was tested in vitro by monitoring the kinetics of conjugated diene formation induced by the free radical generator 2,2′-azobis (amidinopropane) dihydrochloride (AAPH).Their fatty acid composition and the vitamin E content were also measured. Results: The results showed a significantly greater peroxidation rate in cases than in controls (p-0.003), whereas no significant difference was found for the lag phase. The peroxidation rate for the whole population was significantly correlated with the LDL polyunsaturated fatty acid content (r=0.31, p=0.01). An inverse correlation was also observed between this rate and the 18:1/18:2 ratio in LDL (r=-0.36, p=0.004). A longer lag phase and a higher LDL vitamin E content were found in young controls as compared with the other two groups (p<0.0001). Conclusions: The study suggests that LDL oxidation might play a significant role in atherogenesis. It also indicates that the fatty acid composition of LDL may determine their in vivo oxidizability. © 1998, Medikal Press.
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