Establishment and characterization of the Cuvier's beaked whale (Ziphius cavirostris) myogenic cell line

In this study we present the first in vitro model based on a muscle-derived cell line from a male Cuvier's Beaked whale stranded along the Italian coastline. In the myogenic cell line at T0 stage we performed the growth rate assay, the karyotyping, the CBA chromosome banding, G banding, nucleolar organizer regions and telomere analysis and immunocytochemical analysis. The cell characterization at the T2 and T4 stage included: assessment of fusion index, the ultrastructural analysis and immunocytochemical analysis. The population doubling time was determined to be ∼54 h. The cell fusion index at the T2 stage was 8.2 +/− 5 %, at the T4 stage increased at index 28 +/− 10 %. The karyotype analysis revealed a 2n = 42, XY, two pairs of nucleolus organizer regions (NORs), characteristic CBA-banding and PNA-telomeric regions by FISH-mapping. The immunocytochemical results revealed that we generated a heterogeneous population of myogenic cells. A cell population express β-actin, myosin and vime...