Myofascial junction: Emerging insights into the connection between deep/muscular fascia and muscle

Muscles and fasciae are mutually connected and are influenced by force transmission. However, the anatomical connectivity and histological features of these structures remain unclear. The aim of this study was to assess the evidence for connection between muscles and deep/muscular fasciae. We assessed this relationship in different topographical regions of human cadavers and in mice. The results showed that myofascial junctions (MFJ) were made up of collagen I immune-positive structures occupying an average area of 5.11 +/- 0.81 mu m2, distributed in discrete regions at the interface between muscle and fascia with an average density of 9.7 +/- 2.51 MFJ/mm and an average inclination angle of 35.25 +/- 1.52 degrees. These specialized structures also showed collagen III and HA immunopositivity and the presence of elastic fibers. The human myofascial junction can be visualized, opening emerging insights into the connection between deep/muscular fascia and muscle.