The aim was to study the mitochondrial Ca2+ handling of mitochondria isolated from normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) hearts and to establish a possible correlation with systolic blood pressure (SBP). Mitochondrial swelling after Ca2+ addition, Ca2+-retention capacity (CRC) by calcium green method, and membrane potential (Delta Psi m) were assessed. SBP was 124 +/- 1 (WKY) and 235 +/- 6 mm Hg (SHR). CRC, Ca2+ response and Delta Psi m were lower in SHR than WKY mitochondria. The conclusion is: the more depolarized state of SHR than WRY mitochondria results in an abnormal Ca2+ handling and this event is closely associated with the SBP. (C) 2014 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
Mitochondrial calcium handling in normotensive and spontaneously hypertensive rats: correlation with systolic blood pressure levels
Pardo, Alejandro Ciocci;
2015
Abstract
The aim was to study the mitochondrial Ca2+ handling of mitochondria isolated from normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) hearts and to establish a possible correlation with systolic blood pressure (SBP). Mitochondrial swelling after Ca2+ addition, Ca2+-retention capacity (CRC) by calcium green method, and membrane potential (Delta Psi m) were assessed. SBP was 124 +/- 1 (WKY) and 235 +/- 6 mm Hg (SHR). CRC, Ca2+ response and Delta Psi m were lower in SHR than WKY mitochondria. The conclusion is: the more depolarized state of SHR than WRY mitochondria results in an abnormal Ca2+ handling and this event is closely associated with the SBP. (C) 2014 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
