We present here a new microfluidic device allowing droplet-based Reverse Transcription and Quantitative Polymerase Chain Reaction (RT-qPCR). Our platform integrates in a fully-automated manner, single-cell encapsulation, on-flight self-sorting, lysis, PCR thermal cycling and fluorescent detection on droplets which are continuously flowing in a microchannel. This strategy reaches single-cell sensitivity at high-throughput without limiting the number of samples or genes that can be screened per run (Fig.1).
Continuous-flow droplet microfluidic platform for single-cell RT-qPCR analysis
Ferraro D.
2020
Abstract
We present here a new microfluidic device allowing droplet-based Reverse Transcription and Quantitative Polymerase Chain Reaction (RT-qPCR). Our platform integrates in a fully-automated manner, single-cell encapsulation, on-flight self-sorting, lysis, PCR thermal cycling and fluorescent detection on droplets which are continuously flowing in a microchannel. This strategy reaches single-cell sensitivity at high-throughput without limiting the number of samples or genes that can be screened per run (Fig.1).
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