We report here a new and integrated microfluidic platform allowing droplet-based Reverse Transcription and Quantitative Polymerase Chain Reaction (RT-qPCR). Our platform integrates in a fully-automated manner, single-cell encapsulation, lysis, thermal cycling and fluorescent detection on droplets which are continuously flowing in a microchannel (Fig.1).
Droplet microfluidic platform for continuous-flow single-cell RT-qPCR analysis
Ferraro D.
2018
Abstract
We report here a new and integrated microfluidic platform allowing droplet-based Reverse Transcription and Quantitative Polymerase Chain Reaction (RT-qPCR). Our platform integrates in a fully-automated manner, single-cell encapsulation, lysis, thermal cycling and fluorescent detection on droplets which are continuously flowing in a microchannel (Fig.1).File in questo prodotto:
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