SalmonellaentericaserovarTyphimuriumisanimportantzoonoticgastrointestinalpathogenresponsibleforfoodbornediseaseworldwide.Itisasuccessfulentericpathogenbecauseithasdevelopedvirulencestrategiesallowingittosurviveinahighlyinflamedintestinalenvironmentexploitinginflammationtoovercomecolonizationresistanceprovidedbyintestinalmicrobiota.Inthisstudy,weusedpigletsfeaturinganintactmicrobiota,whichnaturallydevelopgastroenteritis,asmodelforsalmonellosis.WecomparedtheeffectsontheintestinalmicrobiotainducedbyawildtypeandanattenuatedS.Typhimuriuminordertoevaluatewhetherthemodificationsarecorrelatedwiththevirulenceofthestrain.ThisstudyshowedthatSalmonellaaltersmicrobiotainavirulence-dependentmanner.WefoundthatthewildtypeS.Typhimuriuminducedinflammationandareductionofspecificprotectingmicrobiotaspecies(SCFA-producingbacteria)normallyinvolvedinprovidingabarrieragainstpathogens.Boththeseeffectscouldcontributetoimpaircolonizationresistance,increasingthehostsusceptibilitytowildtypeS.Typhimuriumcolonization.Incontrast,theattenuatedS.Typhimurium,whichischaracterizedbyareducedabilitytocolonizetheintestine,andbyaverymildinflammatoryresponse,wasunabletosuccessfullysustaincompetitionwiththemicrobiota.
Salmonella enterica serovar typhimurium exploits inflammation to modify swine intestinal microbiota
Drumo, RosannaWriting – Original Draft Preparation
;Gabai, GianfrancoMembro del Collaboration Group
;
2016
Abstract
SalmonellaentericaserovarTyphimuriumisanimportantzoonoticgastrointestinalpathogenresponsibleforfoodbornediseaseworldwide.Itisasuccessfulentericpathogenbecauseithasdevelopedvirulencestrategiesallowingittosurviveinahighlyinflamedintestinalenvironmentexploitinginflammationtoovercomecolonizationresistanceprovidedbyintestinalmicrobiota.Inthisstudy,weusedpigletsfeaturinganintactmicrobiota,whichnaturallydevelopgastroenteritis,asmodelforsalmonellosis.WecomparedtheeffectsontheintestinalmicrobiotainducedbyawildtypeandanattenuatedS.Typhimuriuminordertoevaluatewhetherthemodificationsarecorrelatedwiththevirulenceofthestrain.ThisstudyshowedthatSalmonellaaltersmicrobiotainavirulence-dependentmanner.WefoundthatthewildtypeS.Typhimuriuminducedinflammationandareductionofspecificprotectingmicrobiotaspecies(SCFA-producingbacteria)normallyinvolvedinprovidingabarrieragainstpathogens.Boththeseeffectscouldcontributetoimpaircolonizationresistance,increasingthehostsusceptibilitytowildtypeS.Typhimuriumcolonization.Incontrast,theattenuatedS.Typhimurium,whichischaracterizedbyareducedabilitytocolonizetheintestine,andbyaverymildinflammatoryresponse,wasunabletosuccessfullysustaincompetitionwiththemicrobiota.
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