Aequorin is a Ca(2+) sensitive photoprotein suitable to measure intracellular Ca(2+) transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca(2+) uptake and release of different intracellular organelles. Here, we describe how to use this probe to measure cytosolic Ca(2+) levels and mitochondrial Ca(2+) uptake in mammalian cells.
Ca(2+) Measurements in Mammalian Cells with Aequorin-based Probes
TOSATTO, ANNA;RIZZUTO, ROSARIO;MAMMUCARI, CRISTINA
2017
Abstract
Aequorin is a Ca(2+) sensitive photoprotein suitable to measure intracellular Ca(2+) transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca(2+) uptake and release of different intracellular organelles. Here, we describe how to use this probe to measure cytosolic Ca(2+) levels and mitochondrial Ca(2+) uptake in mammalian cells.
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