Generalised unitarity for dimensionally regulated amplitudes within FDF

We review the Four-Dimensional-Formulation variant of the Four-Dimensional-Helicity scheme, by showing two applications of this regularisation scheme. The first one is the computation of one-loop helicity amplitudes, for which we present preliminary results for the analytic expressions of the one-loop Higgs plus five- gluon amplitudes. In the second part, we study the Colour-Kinematics duality for off-shell diagrams in gauge theories coupled to matter, showing in a diagrammatic way that the Jacobi relations for the kinematic numerators of off-shell diagrams, built with Feynman rules in axial gauge, reduce to definite set of violating terms due to the contributions of sub-graphs