The Cytochrome P4501A (CYP1A) induction is widely employed for environmental biomonitoring, especially in marine organisms. In the present study, the levels of CYP1A was evaluated in the fish species Zosterisessor ophiocephalus sampled in three different sites (Porto Marghera, Val di Brenta and Caroman) of the Venice Lagoon. The expression of CYP1A mRNA, evaluated by Real-Time PCR, was significantly higher in both males and females from the Porto Marghera site. By immunohistochemistry, cellular localization of CYP1A was detected in liver, kidney and ovary. In liver, the strongest immunopositivity was found in males from all sites, whereas the detoxified fish exhibited a faint immunoreactivity. By Western blot, the CYP1A antiserum recognized in the liver a band corresponding to the CYP1A protein. The antibody strongly reacted in males from all sites excepted for control group, whereas weakly reacted in females revealing a marked sex differences in response to environmental pollution. In all sites, the CYP1A gene expression and its protein was increased as evidenced by Real-Time PCR results, as well as by Western blotting and IHC. These results indicated that pollutants are bio-available as evidenced by the biological study and may have an harmful effects on aquatic organisms such as Z. ophiocephalus.
Cytochrome P4501A (CYP1A) as a biomarker to evaluate the environmental quality in Venice lagoon.
POLTRONIERI, CARLO;NEGRATO, ELENA;BERTOTTO, DANIELA;RADAELLI, GIUSEPPE
2011
Abstract
The Cytochrome P4501A (CYP1A) induction is widely employed for environmental biomonitoring, especially in marine organisms. In the present study, the levels of CYP1A was evaluated in the fish species Zosterisessor ophiocephalus sampled in three different sites (Porto Marghera, Val di Brenta and Caroman) of the Venice Lagoon. The expression of CYP1A mRNA, evaluated by Real-Time PCR, was significantly higher in both males and females from the Porto Marghera site. By immunohistochemistry, cellular localization of CYP1A was detected in liver, kidney and ovary. In liver, the strongest immunopositivity was found in males from all sites, whereas the detoxified fish exhibited a faint immunoreactivity. By Western blot, the CYP1A antiserum recognized in the liver a band corresponding to the CYP1A protein. The antibody strongly reacted in males from all sites excepted for control group, whereas weakly reacted in females revealing a marked sex differences in response to environmental pollution. In all sites, the CYP1A gene expression and its protein was increased as evidenced by Real-Time PCR results, as well as by Western blotting and IHC. These results indicated that pollutants are bio-available as evidenced by the biological study and may have an harmful effects on aquatic organisms such as Z. ophiocephalus.
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