A novel vesicular nanosystem was assembled by using pH responsive block copolymers for tumor targeting and intracellular drug release. A poly(gliceroyl-methacrylate-co-C6-imidazoyl-methacryla-te)-mPEG tri- block copolymer was synthesized by RAFT polymerization starting from a mPEG1900-derived chain-transfer agent. This copolymer spontaneously assembles into vesicles at pH 7.4 and rapidly dissociates under conditions that mimics the endosomal compartments, at pH 5. Kinetic stability studies showed that the carrier is stable for 6 hours at pH 7.4 in different conditions. The carrier was efficiently loaded with hydrophilic and hydrophobic fluorescent probes for further in vitro studies to prove active targeting to tumor cells.
New pH Responsive Nanovesicular Vectors For Tumor Targeting
SALMASO, STEFANO;MASTROTTO, FRANCESCA;BERSANI, SARA;CALICETI, PAOLO
2012
Abstract
A novel vesicular nanosystem was assembled by using pH responsive block copolymers for tumor targeting and intracellular drug release. A poly(gliceroyl-methacrylate-co-C6-imidazoyl-methacryla-te)-mPEG tri- block copolymer was synthesized by RAFT polymerization starting from a mPEG1900-derived chain-transfer agent. This copolymer spontaneously assembles into vesicles at pH 7.4 and rapidly dissociates under conditions that mimics the endosomal compartments, at pH 5. Kinetic stability studies showed that the carrier is stable for 6 hours at pH 7.4 in different conditions. The carrier was efficiently loaded with hydrophilic and hydrophobic fluorescent probes for further in vitro studies to prove active targeting to tumor cells.
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