Biochemical pattern of rat liver during and after treatment with 1,2-dichloropropane

1,2-Dichloropropane (1,2 D) induces liver acute toxicity. In chronic experimental treatment of rats, biochemical and histological pattern shows a hyperplastic damage of the liver cell. The present work studies the behaviour of the liver metabolic pattern during and after treatment with 1,2 D. Male, albino, Wistar rats, starting weight 200±10 grams, were treated i.p. for 4 weeks (5 days/week, 2 days without treatment) with 0 and 250 mg/kg b.w. of 1,2 D dissolved in corn oil. The rats were sacrificed after 5, 10, 15 and 20 treatments, and two months after suspension of the treatment. After the sacrifice, the liver was drawed and prepared for determination of Cytochrome P-450 activity (Cyt P-450), reduced glutathione content (GSH), and glutathione S-transferases activity (GST). GSH content shows a statistically significant increase after ten treatments, GST activity significantly increases after 15 treatments, and Cyt P-450 significantly decreases after 20 treatments. Phase II components of liver metabolism are then firstly involved by 1,2 D. The biochemical pattern is indicative of a hyperplastic evolution of the liver cell as it is confirmed by histology. Suspension of the treatment shows a return to normal values of Phase I and Phase II components. These results shows after the suspension of the treatment a tendency to repair of the lesions induced by 1,2 D.