High titer viral supernatants have been photomodified by UVA light (20 kT m(-2)) in the presence of different concentrations of two psolarens (8-methoxypsoralen, 8-MOP and 4,5',8-trimethylpsoralen, TMP) and one angelicin (4,6,4'-trimethylangelicin, TMA). At low concentrations (100-250 ng ml(-1)) 8-MOP and TMA did not show any significant antiviral activity, while TMP demonstrated a reduction of virus infectivity by one log at 250 ng ml(-1). At the highest concentration (5 mu g ml(-1)), TMA and TMP reduced the virus titer by one and more than two logs, respectively, being, therefore, two and four times more active than 8-MOP. The most active compound, TMP, was further tested on HIV-1 viral supernatants. Total inactivation of the HIV-1 (200 SFU) was obtained in the presence of 1 mu g ml(-1) of TMP and 20 kJ m(-2) of UVA light.
Antiretroviral activity of furocoumarins plus UVA light detected by a replication-defective retrovirus
MIOLO, GIORGIA;TOMANIN, ROSELLA;DE ROSSI, ANITA;DALL'ACQUA, FRANCESCO;ZACCHELLO, FRANCO;SCARPA, MAURIZIO
1994
Abstract
High titer viral supernatants have been photomodified by UVA light (20 kT m(-2)) in the presence of different concentrations of two psolarens (8-methoxypsoralen, 8-MOP and 4,5',8-trimethylpsoralen, TMP) and one angelicin (4,6,4'-trimethylangelicin, TMA). At low concentrations (100-250 ng ml(-1)) 8-MOP and TMA did not show any significant antiviral activity, while TMP demonstrated a reduction of virus infectivity by one log at 250 ng ml(-1). At the highest concentration (5 mu g ml(-1)), TMA and TMP reduced the virus titer by one and more than two logs, respectively, being, therefore, two and four times more active than 8-MOP. The most active compound, TMP, was further tested on HIV-1 viral supernatants. Total inactivation of the HIV-1 (200 SFU) was obtained in the presence of 1 mu g ml(-1) of TMP and 20 kJ m(-2) of UVA light.
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