1,2- 14C (2-chloroethyl)phosphonic acid ( 14C-CEPA) translocation and metabolism was studied in short-term experiments on 'Andross' peach trees at the end of Stage I of fruit development. The chemical was applied to the fruit surface or to the abaxial surface of the basal leaf of a developing shoot. When the tracer was applied to the fruit, no translocation of the chemical occurred. When the 14C-CEPA was applied to the leaf, translocation was observed. Thin-layer chromatography (TLC) of the radioactivity recovered in acidic methanol extracts of both fruits and one-year shoots, collected 24, 48 and 96 h after 14C-CEPA application to the leaf, indicated that the translocation occurred in forms different from the original chemical. Treatment of chromatograms with AgNO 3 and FeCl 3 at 100°C showed that the colour reaction of sugars was associated with the radioactive areas. Therefore it seems likely that the translocation of 14C-CEPA occurred in sugar-conjugated form. Results of the TLC of 14C-CEPA before spotting or overspotted with sugars or fruit and leaf extracts, showed that the binding of 14C-CEPA to sugars is not a metabolic reaction, although the complex, being more stable than the original chemical and biologically active, could have physiological significance in determining long-term responses of tissues to treatments with CEPA. © 1981.
Metabolism and translocation of 1, 2- 14C(2-chloroethyl) phosphonic acid in Prunus persica (L.) Batsch
GIULIVO, CLAUDIO;RAMINA, ANGELO;
1981
Abstract
1,2- 14C (2-chloroethyl)phosphonic acid ( 14C-CEPA) translocation and metabolism was studied in short-term experiments on 'Andross' peach trees at the end of Stage I of fruit development. The chemical was applied to the fruit surface or to the abaxial surface of the basal leaf of a developing shoot. When the tracer was applied to the fruit, no translocation of the chemical occurred. When the 14C-CEPA was applied to the leaf, translocation was observed. Thin-layer chromatography (TLC) of the radioactivity recovered in acidic methanol extracts of both fruits and one-year shoots, collected 24, 48 and 96 h after 14C-CEPA application to the leaf, indicated that the translocation occurred in forms different from the original chemical. Treatment of chromatograms with AgNO 3 and FeCl 3 at 100°C showed that the colour reaction of sugars was associated with the radioactive areas. Therefore it seems likely that the translocation of 14C-CEPA occurred in sugar-conjugated form. Results of the TLC of 14C-CEPA before spotting or overspotted with sugars or fruit and leaf extracts, showed that the binding of 14C-CEPA to sugars is not a metabolic reaction, although the complex, being more stable than the original chemical and biologically active, could have physiological significance in determining long-term responses of tissues to treatments with CEPA. © 1981.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
