The authors present a gas chromatographic method which allows for the rapid identification and assay of barbiturates and antiepileptics in body fluids. The Kupferberger procedure for extraction is used. The identification is obtained on 2 different columns: 10% Dexil 300 GC on Chromosorb W HP and 3% OV 17 on Chromosorb G AW DMCS (80-100 mesh). A considerable and interesting reduction of the barbiturates adsorption in the columns is obtained by preliminary conditioning with tetraethylorthosilicate. In this way, phenobarbital and cyclobarbital may also be assayed up to 1 μg/ml in blood. For the simultaneous assay of therapeutic levels of phenobarbital and hydantoins methyl derivatives are better employed: blood concentrations as low as 0.1 μg/ml are easily detected.
A sensitive method for gas chromatographic assay of barbiturates in body fluids
FERRARA, SANTO;
1977
Abstract
The authors present a gas chromatographic method which allows for the rapid identification and assay of barbiturates and antiepileptics in body fluids. The Kupferberger procedure for extraction is used. The identification is obtained on 2 different columns: 10% Dexil 300 GC on Chromosorb W HP and 3% OV 17 on Chromosorb G AW DMCS (80-100 mesh). A considerable and interesting reduction of the barbiturates adsorption in the columns is obtained by preliminary conditioning with tetraethylorthosilicate. In this way, phenobarbital and cyclobarbital may also be assayed up to 1 μg/ml in blood. For the simultaneous assay of therapeutic levels of phenobarbital and hydantoins methyl derivatives are better employed: blood concentrations as low as 0.1 μg/ml are easily detected.
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