Rabbit antiserum raised against purified maize polyamine oxidase (MPAO) recognises numerous proteins of different plant extracts in immunoblotting. In order to render this antiserum specific, it was submitted to an affinity chromatography procedure, using as immunoadsorbent MPAO blotted onto nitrocellulose and deglycosylated with sodium metaperiodate. Affinity purified antibodies eluted by MgCl2, tested by immunoblotting and ELISA, showed an improved specificity for the enzyme and a dramatic reduction of cross-reactivities with other plant components, thus suggesting that this procedure is suitable for the preparation of antibodies to be used in immunohistochemical studies.
Immunological cross reactivity of plant glycoproteins: maize polyamine oxidase, a cell wall glycoprotein.
RAIOLA, ALESSANDRO;
1995
Abstract
Rabbit antiserum raised against purified maize polyamine oxidase (MPAO) recognises numerous proteins of different plant extracts in immunoblotting. In order to render this antiserum specific, it was submitted to an affinity chromatography procedure, using as immunoadsorbent MPAO blotted onto nitrocellulose and deglycosylated with sodium metaperiodate. Affinity purified antibodies eluted by MgCl2, tested by immunoblotting and ELISA, showed an improved specificity for the enzyme and a dramatic reduction of cross-reactivities with other plant components, thus suggesting that this procedure is suitable for the preparation of antibodies to be used in immunohistochemical studies.
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