The present research investigates the occurrence of hyaline droplet (HD) accumulation related to age, dose, and time after treatment in male Wistar rats given a single i.p. injection of hexachloro-1:3-butadiene (HCBD). In the study on age, rats from 1 to 12 months of age were treated with 100 mg/kg b.w. HCBD dose. Rats treated at 2 months of age showed a greater accumulation of HD than the other age groups; HD accumulation was not observed in 1 month aged rats. In the dose-response study, the treatment with 25, 50, and 100 mg/kg b.w. at 2 months of age caused HD accumulation in the proximal convoluted tubule at all doses, with 100 mg/kg b.w. group slightly more affected.. Finally, in the time-course study, rats treated with a 100 mg/kg b.w. dose at 2 months of age and sacrificed at 6, 12, 24, 48, 72, and 96 hours post-dosing showed a time-related HD accumulation in term of incidence and severity, after 6 hours, with a peak at 24 and 48 hours and decreasing at 72 hours and 96 hours. The present results show that HD accumulation is an early finding and is unrelated to dose level and particularly evident in rats of 2 month of age. These findings in male rats treated with HCBD emphasized the importance of considering the age of rats at the start of a study the more sensitive model is used in the detection of nephrotoxic effects of chemicals.
Hyaline droplet accumulation in kidney of rats treated with hexachloro-1:3-butadiene: influence of age, dose, and time-course
MONGILLO, MICHELE;BARTOLUCCI, GIOVANNI BATTISTA;CHIARA, FEDERICA;TREVISAN, ANDREA
2013
Abstract
The present research investigates the occurrence of hyaline droplet (HD) accumulation related to age, dose, and time after treatment in male Wistar rats given a single i.p. injection of hexachloro-1:3-butadiene (HCBD). In the study on age, rats from 1 to 12 months of age were treated with 100 mg/kg b.w. HCBD dose. Rats treated at 2 months of age showed a greater accumulation of HD than the other age groups; HD accumulation was not observed in 1 month aged rats. In the dose-response study, the treatment with 25, 50, and 100 mg/kg b.w. at 2 months of age caused HD accumulation in the proximal convoluted tubule at all doses, with 100 mg/kg b.w. group slightly more affected.. Finally, in the time-course study, rats treated with a 100 mg/kg b.w. dose at 2 months of age and sacrificed at 6, 12, 24, 48, 72, and 96 hours post-dosing showed a time-related HD accumulation in term of incidence and severity, after 6 hours, with a peak at 24 and 48 hours and decreasing at 72 hours and 96 hours. The present results show that HD accumulation is an early finding and is unrelated to dose level and particularly evident in rats of 2 month of age. These findings in male rats treated with HCBD emphasized the importance of considering the age of rats at the start of a study the more sensitive model is used in the detection of nephrotoxic effects of chemicals.
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