An isocratic high-performance liquid chromatography (HPLC) methodwas developed and validated to determine Aloe Emodin (AE) in mouse plasma. The analysis required 0.3 ml of plasma and involves extraction with dichloromethane. The HPLC separationwas carried out on Symmetry Shield RP18, a mobile phase of methanol–water–acetic acid (65:35:0.2) and fluorescence detection at λex = 410 nm and λem = 510 nm. The retention time of AEwas 11.7 min. The assaywas linear from 10 to 1000 ng/ml (r2 ≥ 0.999), showed intra- and inter-day precision within 7.8 and 4.7%, and accuracy of 87.3–105.7%. Detection limit (LOD) and quantification limit (LOQ) were 4.5 and 5 ng/ml, respectively. The method was applied to determine for the first time the pharmacokinetic of AE in mice.
High-performance liquid chromatographic assay for the determination of Aloe Emodin in mouse plasma
MUCIGNAT, CARLA;PECERE, TERESA;ZAGOTTO, GIUSEPPE;
2003
Abstract
An isocratic high-performance liquid chromatography (HPLC) methodwas developed and validated to determine Aloe Emodin (AE) in mouse plasma. The analysis required 0.3 ml of plasma and involves extraction with dichloromethane. The HPLC separationwas carried out on Symmetry Shield RP18, a mobile phase of methanol–water–acetic acid (65:35:0.2) and fluorescence detection at λex = 410 nm and λem = 510 nm. The retention time of AEwas 11.7 min. The assaywas linear from 10 to 1000 ng/ml (r2 ≥ 0.999), showed intra- and inter-day precision within 7.8 and 4.7%, and accuracy of 87.3–105.7%. Detection limit (LOD) and quantification limit (LOQ) were 4.5 and 5 ng/ml, respectively. The method was applied to determine for the first time the pharmacokinetic of AE in mice.
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