AFLP fingerprinting tecnique has been used to enrich existing genetic maps in plants, bacteria and less widely also in animal genomes. Aim of this study was to explore the use of biallelic Amplified Fragment Length Polymorphism (AFLP) markers in a local poultry breed reared in the Veneto region (Italy). Genomic DNA was extracted from whole blood obtained from 12 cocks belonging to Ermellinata di Rovigo breed. AFLP genotyping was carried out using four enzime combinations (EcoRI/TaqI, EcoRI/MseI, PstI/TaqI, PstI/MseI), following the protocol described by Barcaccia et al.1999 . Results showed that the use of MseI is not appropriate in the breed examined because it doesn’t generate polimorphic profiles, while TaqI appears more favourable. The highest number of polymorphic bands is obtained using TaqI and EcoRI, but further experimentation will be necessary to choose the appropriate endonucleases, as only one primer combination for each pair of enzimes has been tested. This study showed the applicability of AFLP markers to the characterisation of the poultry breed examined. Further tests will permit to validate a definitive experimental protocol that might be used also for other poultry breeds.
Use of AFLP markers to genome mapping in local poultry breeds
DE MARCHI, MASSIMO;CASSANDRO, MARTINO;BARCACCIA, GIANNI;RAMANZIN, MAURIZIO;
2003
Abstract
AFLP fingerprinting tecnique has been used to enrich existing genetic maps in plants, bacteria and less widely also in animal genomes. Aim of this study was to explore the use of biallelic Amplified Fragment Length Polymorphism (AFLP) markers in a local poultry breed reared in the Veneto region (Italy). Genomic DNA was extracted from whole blood obtained from 12 cocks belonging to Ermellinata di Rovigo breed. AFLP genotyping was carried out using four enzime combinations (EcoRI/TaqI, EcoRI/MseI, PstI/TaqI, PstI/MseI), following the protocol described by Barcaccia et al.1999 . Results showed that the use of MseI is not appropriate in the breed examined because it doesn’t generate polimorphic profiles, while TaqI appears more favourable. The highest number of polymorphic bands is obtained using TaqI and EcoRI, but further experimentation will be necessary to choose the appropriate endonucleases, as only one primer combination for each pair of enzimes has been tested. This study showed the applicability of AFLP markers to the characterisation of the poultry breed examined. Further tests will permit to validate a definitive experimental protocol that might be used also for other poultry breeds.
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