A new alkyl maleimido activated monomethoxypoly(ethylene glycol) (PEG-C18-Mal) for site-directed protein conjugation was synthesised and characterized. As compared to the commercial mPEG-maleimido, PEG-C18-Mal was more efficient in the bioconjugation to 17Cys of rh-G-CSF in guanidinium free buffer and gave more stable bioconjugate. Also, the new bioconjugate displayed high biological activity.
Effective G-CSF Site Directed PEGylation of Hindered Aminoacids.
SALMASO, STEFANO;SCOMPARIN, ANNA;BERSANI, SARA;CALICETI, PAOLO
2007
Abstract
A new alkyl maleimido activated monomethoxypoly(ethylene glycol) (PEG-C18-Mal) for site-directed protein conjugation was synthesised and characterized. As compared to the commercial mPEG-maleimido, PEG-C18-Mal was more efficient in the bioconjugation to 17Cys of rh-G-CSF in guanidinium free buffer and gave more stable bioconjugate. Also, the new bioconjugate displayed high biological activity.
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