Four MHCs can be expressed in adult skeletal muscles of mammals. The slow, 2A and 2X isoforms have been found expressed in all species examined, whereas the 2B isoform has been so far detected only in laboratory mammals and marsupials. Recent works (Chang et al., 1997; Toniolo et al., 2004) showed the expression of 2B MHC also in pig skeletal muscles as well as in specialized eye muscles as retractor bulbi and rectus lateralis. In the cow, MHC-2B isoform is expressed only in those specialized muscles (Maccatrozzo et al., 2004). The issue about MHC isoforms expressed in fast or type 2 dog fibres is still open (Snow et al., 1982; Strbenc et al., 2004). Immunohistochemistry, protein electrophoresis and RT-PCR showed that type 2 dog fibres contain the MHC-2X isoform both in ‘‘hybrid’’ 2A/X and in ‘‘pure’’ 2X fibres. The limb and trunk muscles express only 1, 2A and 2X-MHC isoforms, whereas the MHC-2B isoform was present only in specialized eye muscles and probably in thyroarytenoideus muscle (Wu et al., 2000). The 2B isoform of dog and cow is a muscle specific protein (allotype), as for 2 M and 2EO MHCs?
Dog myosin heavy chain gene isoform composition:comparison between limb and specialised muscles
MASCARELLO, FRANCESCO;MACCATROZZO, LISA;PATRUNO, MARCO VINCENZO;TONIOLO, LUANA;REGGIANI, CARLO
2005
Abstract
Four MHCs can be expressed in adult skeletal muscles of mammals. The slow, 2A and 2X isoforms have been found expressed in all species examined, whereas the 2B isoform has been so far detected only in laboratory mammals and marsupials. Recent works (Chang et al., 1997; Toniolo et al., 2004) showed the expression of 2B MHC also in pig skeletal muscles as well as in specialized eye muscles as retractor bulbi and rectus lateralis. In the cow, MHC-2B isoform is expressed only in those specialized muscles (Maccatrozzo et al., 2004). The issue about MHC isoforms expressed in fast or type 2 dog fibres is still open (Snow et al., 1982; Strbenc et al., 2004). Immunohistochemistry, protein electrophoresis and RT-PCR showed that type 2 dog fibres contain the MHC-2X isoform both in ‘‘hybrid’’ 2A/X and in ‘‘pure’’ 2X fibres. The limb and trunk muscles express only 1, 2A and 2X-MHC isoforms, whereas the MHC-2B isoform was present only in specialized eye muscles and probably in thyroarytenoideus muscle (Wu et al., 2000). The 2B isoform of dog and cow is a muscle specific protein (allotype), as for 2 M and 2EO MHCs?
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