Blood samples from cattle from herds of the province of Venice, were submitted to microbiological investigation to detect Bartonella spp. after a suspect of infection, based on microscopic observation of blood smears. Blood culture were performed and 10 samples resulted positive. DNA extracted from colonies isolated from each of the 10 culture-positive samples, was subjected to PCR amplifying the16S-23S rRNA intergenic region (ITS), and the genes citrate synthase (gltA), RNA Polymerase β-subunit (rpoB), Riboflavin synthase (ribC) and further sequenced. The sequences obtained by BLAST showed percentages of homology with reference B. bovis sequences ranging from 98% to 100%. This is the first report of B. bovis in Italy.
Primo isolamento di Bartonella Bovis in ruminanti domestici in Italia.
MENANDRO, MARIA LUISA;MONDIN, ALESSANDRA;PASOTTO, DANIELA;DRIGO, MICHELE;MARTINI, MARCO
2006
Abstract
Blood samples from cattle from herds of the province of Venice, were submitted to microbiological investigation to detect Bartonella spp. after a suspect of infection, based on microscopic observation of blood smears. Blood culture were performed and 10 samples resulted positive. DNA extracted from colonies isolated from each of the 10 culture-positive samples, was subjected to PCR amplifying the16S-23S rRNA intergenic region (ITS), and the genes citrate synthase (gltA), RNA Polymerase β-subunit (rpoB), Riboflavin synthase (ribC) and further sequenced. The sequences obtained by BLAST showed percentages of homology with reference B. bovis sequences ranging from 98% to 100%. This is the first report of B. bovis in Italy.
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