A method based on FIA-ESI-MS has been developed to profile the major constituents of Hypericum perforatum extracts. The objective was to obtain simultaneous semi-quantitative data on hypericin, chlorogenic acid, rutin, quercitrin, quercetin and hyperforin contents for a high-throughput screening of the raw plant material. The principal drawback of FIA-ESI analysis of complex mixtures involves ion suppression effects: the ionization of some components of the mixture can be severely suppressed by ionization of others. The results show that this problem can be alleviated using a new approach to generate calibration curves.
A Semi-quantitative FIA-ESI-MS Method for the Rapid Screening of Hypericum perforatum Crude Extracts
PIOVAN, ANNA;FILIPPINI, RAFFAELLA;CANIATO, ROSAMARIA
2010
Abstract
A method based on FIA-ESI-MS has been developed to profile the major constituents of Hypericum perforatum extracts. The objective was to obtain simultaneous semi-quantitative data on hypericin, chlorogenic acid, rutin, quercitrin, quercetin and hyperforin contents for a high-throughput screening of the raw plant material. The principal drawback of FIA-ESI analysis of complex mixtures involves ion suppression effects: the ionization of some components of the mixture can be severely suppressed by ionization of others. The results show that this problem can be alleviated using a new approach to generate calibration curves.
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