Timely diagnosis of mixed lineage leukemia (MLL) rearrangements in pediatric patients with B-cell precursor acute lymphoblastic leukemia (BP-ALL) is highly relevant in patient stratification. The presence of t(4;11)-MLL rearrangement is the most important criteria for high-risk stratification in protocols for childhood precursor BP-ALL. Current tests to identify t(4;11)-MLL involve karyotyping, fluorescence in situ hybridization and reverse transcription-PCR. The tests, however, are expensive both in terms of personnel and reagents. They do not provide a fast diagnosis and may fail due to technical reasons or high variability in the breakpoint that does not allow for standard reverse transcription-PCR detection. Therefore, a fast and reliable test that identifies MLL rearranged BP-ALL is highly welcome, particularly in low-income countries.
Validation of NG2 antigen in identifying BP-ALL patients with MLL rearrangements using qualitative and quantitative flow cytometry: a prospective study
TE KRONNIE, GEERTRUDY;BASSO, GIUSEPPE
2008
Abstract
Timely diagnosis of mixed lineage leukemia (MLL) rearrangements in pediatric patients with B-cell precursor acute lymphoblastic leukemia (BP-ALL) is highly relevant in patient stratification. The presence of t(4;11)-MLL rearrangement is the most important criteria for high-risk stratification in protocols for childhood precursor BP-ALL. Current tests to identify t(4;11)-MLL involve karyotyping, fluorescence in situ hybridization and reverse transcription-PCR. The tests, however, are expensive both in terms of personnel and reagents. They do not provide a fast diagnosis and may fail due to technical reasons or high variability in the breakpoint that does not allow for standard reverse transcription-PCR detection. Therefore, a fast and reliable test that identifies MLL rearranged BP-ALL is highly welcome, particularly in low-income countries.
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