Under phosphate deprivation, higher plants change their lipid composition and recycle phosphate from phospholipids. A phospholipase D, PLD zeta 2, is involved in this recycling and in other cellular functions related to plant development. We investigated the localization of Arabidopsis PLD zeta 2 by cell fractionation and in vivo GFP confocal imaging. AtPLD zeta 2 localizes to the tonoplast and the Nter regulatory domain is sufficient for its sorting. Under phosphate deprivation, AtPLD zeta 2 remains located in the tonoplast but its distribution is uneven. We observed PLD zeta 2-enriched tonoplast domains preferentially positioned close to mitochondria and beside chloroplasts. In absence of PLD zeta 2, membrane developments were visualized inside vacuoles.
Phosphate availability affects the tonoplast localization of PLD 2, an Arabidopsis thaliana phospholipase D
BALDAN, BARBARA;
2008
Abstract
Under phosphate deprivation, higher plants change their lipid composition and recycle phosphate from phospholipids. A phospholipase D, PLD zeta 2, is involved in this recycling and in other cellular functions related to plant development. We investigated the localization of Arabidopsis PLD zeta 2 by cell fractionation and in vivo GFP confocal imaging. AtPLD zeta 2 localizes to the tonoplast and the Nter regulatory domain is sufficient for its sorting. Under phosphate deprivation, AtPLD zeta 2 remains located in the tonoplast but its distribution is uneven. We observed PLD zeta 2-enriched tonoplast domains preferentially positioned close to mitochondria and beside chloroplasts. In absence of PLD zeta 2, membrane developments were visualized inside vacuoles.
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