The herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) was tested on mitochondria from etiolated pea (Pisum safivum 1. cv Alaska) stems. This compound when used at micromolar concentrations (-20 p ~ in)hi bited malate- and succinate-dependent respiration by intad mitochondria but not oxidation of exogenously added NADH. Bromoxynil did not affed the adivities of the succinic and the internal NADH dehydrogenases. Analyses of the effects induced by this herbicide on the membrane potential, ApH, matrix Caz+ movements, and dicarboxylate transport demonstrated that bromoxynil is likely to a d as an inhibitor of the dicarboxylate carrier. In addition, bromoxynil caused a mild membrane uncoupling at concentrations 220 p ~ N.o effed on the ATPase adivity was observed.
EFFECTS OF 3,5-DIBROMO-4-HYDROXYBENZONITRILE (BROMOXYNIL) ON BIOENERGETICS OF HIGHER-PLANT MITOCHONDRIA (PISUM-SATIVUM)
ZOTTINI, MICHELA;
1994
Abstract
The herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) was tested on mitochondria from etiolated pea (Pisum safivum 1. cv Alaska) stems. This compound when used at micromolar concentrations (-20 p ~ in)hi bited malate- and succinate-dependent respiration by intad mitochondria but not oxidation of exogenously added NADH. Bromoxynil did not affed the adivities of the succinic and the internal NADH dehydrogenases. Analyses of the effects induced by this herbicide on the membrane potential, ApH, matrix Caz+ movements, and dicarboxylate transport demonstrated that bromoxynil is likely to a d as an inhibitor of the dicarboxylate carrier. In addition, bromoxynil caused a mild membrane uncoupling at concentrations 220 p ~ N.o effed on the ATPase adivity was observed.Pubblicazioni consigliate
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