The effect of different DNA sequences on the photoreaction of various furocoumarin derivatives was investigated from a quantitative poin of view using a number of selfcomplementary oligonucleotides. The furocoumarins included classical bifunctional derivatives, such as 8-methoxy and 5-methoxypsoralen, as well as monofunctional compounds, such as angelicin and benzopsoralen. This approach, in combination with footprinting studies, appears to be quite useful for a quantitative investigation of the process of covalent binding of psoralens to specific sites in DNA.
Sequence specificity of psoralen photobinding to DNA: a quantitative approach
GIA, ORNELLA MARIA;PALUMBO, MANLIO
1992
Abstract
The effect of different DNA sequences on the photoreaction of various furocoumarin derivatives was investigated from a quantitative poin of view using a number of selfcomplementary oligonucleotides. The furocoumarins included classical bifunctional derivatives, such as 8-methoxy and 5-methoxypsoralen, as well as monofunctional compounds, such as angelicin and benzopsoralen. This approach, in combination with footprinting studies, appears to be quite useful for a quantitative investigation of the process of covalent binding of psoralens to specific sites in DNA.
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