Small pieces of gastrointestinal tract mucosa were collected from 20 young bulls 18 months old. Samples were fixed in 10% formalin with 2% calcium acetate added and in B4G, embedded in paraffin and serially sectioned. The slides were stained with PAS, Alcian blue (pH 2.5), HID/AB (Spicer, 1965). Oligocaccharidic side chains were investigated by means of five biotinylated lectins (DBA, PNA, WGA succinylated form, UEA-I and LPA). The histochemical methods employed pointed out that secretion elaborated from the different investigated glands exhibits similar composition. Pyloric glands, terminal parts of Brunner’s glands and goblet cells in the cattle elaborate both neutral and acidic sulphated and carboxylated glycoconjugates. The oligosaccharidic side chains appear rich in GlcNAc and -Lfucose, while exhibit moderate quantity of GalNAc residues; finally DGal(1-3)GalNAc is poorly represented both terminal and penultimate to terminal sialyc acid residues. It is noteworthy that subterminal parts of Brunner’s glands exhibit characteristic saccharidic moiety.
Osservazioni comparative sul secreto delle ghiandole piloriche e duodenali e delle cellule mucipare caliciformi. Indagini istochimiche nel Bovino
VERDIGLIONE, RINA;
2000
Abstract
Small pieces of gastrointestinal tract mucosa were collected from 20 young bulls 18 months old. Samples were fixed in 10% formalin with 2% calcium acetate added and in B4G, embedded in paraffin and serially sectioned. The slides were stained with PAS, Alcian blue (pH 2.5), HID/AB (Spicer, 1965). Oligocaccharidic side chains were investigated by means of five biotinylated lectins (DBA, PNA, WGA succinylated form, UEA-I and LPA). The histochemical methods employed pointed out that secretion elaborated from the different investigated glands exhibits similar composition. Pyloric glands, terminal parts of Brunner’s glands and goblet cells in the cattle elaborate both neutral and acidic sulphated and carboxylated glycoconjugates. The oligosaccharidic side chains appear rich in GlcNAc and -Lfucose, while exhibit moderate quantity of GalNAc residues; finally DGal(1-3)GalNAc is poorly represented both terminal and penultimate to terminal sialyc acid residues. It is noteworthy that subterminal parts of Brunner’s glands exhibit characteristic saccharidic moiety.
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