The basic steps of the 32P-postlabelling assay and details currently used in monitoring mussel DNA adducts are reported. Namely, details are given for DNA purification, enzymatic DNA hydrolysis, DNA adduct enhancement, postlabelling/separation and detection of the DNA adducts.
Detection of bulky aromatic adducts by 32P-postlabelling
VENIER, PAOLA
2001
Abstract
The basic steps of the 32P-postlabelling assay and details currently used in monitoring mussel DNA adducts are reported. Namely, details are given for DNA purification, enzymatic DNA hydrolysis, DNA adduct enhancement, postlabelling/separation and detection of the DNA adducts.
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