A protocol for preserving grape embryogenic cultures indefinitely has been defined, and through recurrent cycles of secondary embryogenesis, Vitis rupestris Scheele cultures are still regenerating after 10 years. The morphogenic competence of a sample of 1,204 somatic embryos with such a long history has been evaluated. Within a 15-month-long culture, secondary embryogenesis regeneration reached an average efficiency of 23%, proving that morphogenic competence is retained during prolonged culture times. While the culture maintenance described here was one of the crucial aspects of our genetic transformation protocol, several V. rupestris plants with various exogenous genes have been regenerated during these last 10 years.
Morphogenic competence of secondary somatic embryos with a long culture story
RASCIO, NICOLETTA
2001
Abstract
A protocol for preserving grape embryogenic cultures indefinitely has been defined, and through recurrent cycles of secondary embryogenesis, Vitis rupestris Scheele cultures are still regenerating after 10 years. The morphogenic competence of a sample of 1,204 somatic embryos with such a long history has been evaluated. Within a 15-month-long culture, secondary embryogenesis regeneration reached an average efficiency of 23%, proving that morphogenic competence is retained during prolonged culture times. While the culture maintenance described here was one of the crucial aspects of our genetic transformation protocol, several V. rupestris plants with various exogenous genes have been regenerated during these last 10 years.Pubblicazioni consigliate
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