Accumulation of poly-3-hydroxybutyrate P(3HB) granules serves to sustain the survival of bacterial cells under conditions of nutrient shortage. P(3HB) is produced by many Rhizobium, Sinorhizobium, Bradyrhizobium and Azorhizobium species in free-living and in symbiotic state. However, the physiol. role for this polymer has not been entirely clarified. A stable P(3HB)-minus mutant of Rhizobium tropici was obtained by inactivation of the phaC gene and characterized. Nodule-inducing ability on bean plants and ARA of the mutant strain did not differ from those of the parental strain. However, P(3HB)-minus mutant showed higher accumulation of glycogen as compared to the parental strain. Persistence studies were performed and survival differences in carbon-free liquid microcosms were observed between the two strains.
Poly-3-hydroxybutyrate has an important role for the survival of Rhizobium tropici under starvation
POVOLO, SILVANA;CASELLA, SERGIO
2004
Abstract
Accumulation of poly-3-hydroxybutyrate P(3HB) granules serves to sustain the survival of bacterial cells under conditions of nutrient shortage. P(3HB) is produced by many Rhizobium, Sinorhizobium, Bradyrhizobium and Azorhizobium species in free-living and in symbiotic state. However, the physiol. role for this polymer has not been entirely clarified. A stable P(3HB)-minus mutant of Rhizobium tropici was obtained by inactivation of the phaC gene and characterized. Nodule-inducing ability on bean plants and ARA of the mutant strain did not differ from those of the parental strain. However, P(3HB)-minus mutant showed higher accumulation of glycogen as compared to the parental strain. Persistence studies were performed and survival differences in carbon-free liquid microcosms were observed between the two strains.
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