Continuous small angle X-ray scattering experiments were performed on high molecular weight proteins, hemocyanins and transferrins, with the Frascati drift chamber area detector and the diffraction camera. The detector used the time structure of Adone synchrotron radiation for operating a faster data acquisition system with real time graphics for monitoring and interpreting scattering data. The diffraction camera with the relative angular resolution DELTA-k/k of 0.12 could measure a minimum scattering momentum k(min) of 8 x 10(-3) angstrom-1. Preliminary results obtained on lyophilized half-met hemocyanin and transferrins indicate formation of clusters. Small angle X-ray scattering data on the native hemocyanin is in agreement with the model (a hollow cylinder) derived from electron microscopy.
APPLICATION OF THE IMPROVED AREA DETECTOR FOR CONTINUOUS SMALL-ANGLE X-RAY-SCATTERING USING SYNCHROTRON RADIATION
BELTRAMINI, MARIANO;
1991
Abstract
Continuous small angle X-ray scattering experiments were performed on high molecular weight proteins, hemocyanins and transferrins, with the Frascati drift chamber area detector and the diffraction camera. The detector used the time structure of Adone synchrotron radiation for operating a faster data acquisition system with real time graphics for monitoring and interpreting scattering data. The diffraction camera with the relative angular resolution DELTA-k/k of 0.12 could measure a minimum scattering momentum k(min) of 8 x 10(-3) angstrom-1. Preliminary results obtained on lyophilized half-met hemocyanin and transferrins indicate formation of clusters. Small angle X-ray scattering data on the native hemocyanin is in agreement with the model (a hollow cylinder) derived from electron microscopy.
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