Atthenonpermissivetemperature,somaticembryogenesisofthetemperature-sensitive(ts)carrotceiimutanttsll does not proceed beyond the globular stage. This developmental arrest can be lifted by the addition of proteins secreted by wild-typecellstotheculturemedium.Fromthismixtureofsecretedproteins,a32-kDglycoprotein,designatedextracel- lular protein 3 (EP3), that allows completion of somatic embryo development in tsll at the nonpermissivetemperature was purified. On the basis of peptide sequences and biochemicai characterization, EP3 was identified as a glycosylated acidicendochitinase.Theadditionofthe32-kDendochitinasetotsll embryoculturesatthenonpermissivetemperature appeared to promote the formation of a correctly formed embryo protoderm. These results imply that a glycosylated acidic endochitinase has an important function in early plant somatic embryo development.
A carrot somatic embryo mutant is rescued by chitinase.
LO SCHIAVO, FIORELLA;TERZI, MARIO;
1992
Abstract
Atthenonpermissivetemperature,somaticembryogenesisofthetemperature-sensitive(ts)carrotceiimutanttsll does not proceed beyond the globular stage. This developmental arrest can be lifted by the addition of proteins secreted by wild-typecellstotheculturemedium.Fromthismixtureofsecretedproteins,a32-kDglycoprotein,designatedextracel- lular protein 3 (EP3), that allows completion of somatic embryo development in tsll at the nonpermissivetemperature was purified. On the basis of peptide sequences and biochemicai characterization, EP3 was identified as a glycosylated acidicendochitinase.Theadditionofthe32-kDendochitinasetotsll embryoculturesatthenonpermissivetemperature appeared to promote the formation of a correctly formed embryo protoderm. These results imply that a glycosylated acidic endochitinase has an important function in early plant somatic embryo development.
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