Toxicity of Tamoxifen on Daphnia pulex

Daphnia pulex is a water flea considered an environmental indicator species. In this experiment we exposed Daphnia to Tamoxifen in low or high concentrations, dissolved in dimethyl sulfoxide with water, and we measured the amount of proteins at day 2 and 7. With the R package maSigPro we selected proteins changing significantly over time among the four experimental groups and we developed a cluster analysis for the behavior of profiles over time, to understand which and how these specific proteins change according to the treatment received. The information obtained from this study represents an important first step towards characterizing patterns specific to environmental contaminants.