Previous analysis of photosynthetic electron transport of isolated thylakoid membranes in iron-deficient sunflower plants showed a decrease in the whole chain and photosystem I (PSI) activities, whereas photosystem II (PSII) activity resulted to be unaffected (Tognini et al., 1996). On the basis of these results, the analysis of the molecular composition of thylakoid membranes was carried out. By using immuno-blotting methods, a decrease in the protein content of PSI in iron-deficient plants was detected, while light-harvesting complex II (LHCII) increased and PSII core complex (CC) did not change compared to the controls. In iron-deficient plants, the tetramethylbenzidine staining of thylakoid protein gels showed a decrease in the content of cytochrome f and cytochrome b6, while cytochrome b559 remained unchanged in control and stressed plants.
Alterations in thylakoid membrane composition induced by iron starvation in sunflower
BALDAN, BARBARA;
2000
Abstract
Previous analysis of photosynthetic electron transport of isolated thylakoid membranes in iron-deficient sunflower plants showed a decrease in the whole chain and photosystem I (PSI) activities, whereas photosystem II (PSII) activity resulted to be unaffected (Tognini et al., 1996). On the basis of these results, the analysis of the molecular composition of thylakoid membranes was carried out. By using immuno-blotting methods, a decrease in the protein content of PSI in iron-deficient plants was detected, while light-harvesting complex II (LHCII) increased and PSII core complex (CC) did not change compared to the controls. In iron-deficient plants, the tetramethylbenzidine staining of thylakoid protein gels showed a decrease in the content of cytochrome f and cytochrome b6, while cytochrome b559 remained unchanged in control and stressed plants.
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